Abstract

Background & Objective: Plants have been tremendously utilized as a source of medicine, therefore this study aimed at investigating the biochemical composition, antimicrobial properties and green synthesis characterization of Murraya koenigi (Curry leaf) using its aqueous extract. Method: Standard methods were used to analyse the various parameters of nutritional, antinutrients, phytochemicals, antioxidants, antimicrobial green synthesis and molecular docking. Results & Conclusion: The results of proximate composition revealed a nutrient-dense profile, it also contained nutritionally valuable minerals while anti-nutrient assessment showed minimal content within the daily recommended allowance. The phytochemical screening of the aqueous and ethanol extracts showed the presence of some bioactive compounds like flavonoids, alkaloids, tannins, and phenol. The antioxidant evaluation demonstrated remarkable free radical scavenging abilities, high content of some antioxidant compounds. Antimicrobial evaluation of the aqueous extract and its synthesized nanoparticles of silver (AgNP), copper (CuNP) and zinc (ZnNP) against a range of bacterial and fungal pathogens showed remarkable inhibitory zones. FTIR analysis of the aqueous extract also revealed the presence of some functional groups such as phenolics, carboxylic acids, and amines while UV/Visible spectrophotometric scanning displayed intensive absorption of light by the inherent chemical substances of the aqueous and synthesised nanoparticle solutions. Bioinformatics approaches identified potential molecular targets for the bioactive compounds. Docking simulations revealed strong binding affinities between key phytochemicals, such as citral, β-myrcene, and geraniol, and the 1Y9R (PDB ID MCR) receptor, demonstrating their potentials as therapeutic agents. Molecular interaction studies further illustrated the compatibility of these compounds with active site residues, reinforcing their drug-likeness properties. Clustering analysis of the major phytochemicals provided insights into their synergistic bioactivity, enhancing the overall therapeutic potentials of the extract.

References

1. Franyoto YD, Nurrochmad A, Fakhrudin N. (2024) Murraya koenigii L. Spreng.: An updated review of chemical composition, pharmacological effects, and toxicity studies. J Appl Pharm Sci.; 14(06):011–027. DOI: 10.7324/JAPS.2024.169254
2. Sangwan N, Kau, R, Pandey S. (2020). Phytochemical and pharmacological properties of curry leaf (Murraya koenigii): A comprehensive review. International Journal of Herbal Medicine, 8(1), 45-56.
3. Mishra S, Awasthi S, Sharma V. (2022). Pharmacological significance of Murraya koenigii: A review of its medicinal properties and applications. Journal of Ethnopharmacology, 287, 115074.
4. Whelton PK, Carey RM, Aronow WS, Casey DE, Collins KJ, Himmelfarb CD. (2018). Guidelines for the prevention, detection, evaluation, and management of high blood pressure in adults. Journal of the American College of Cardiology, 71(19), e127–e248.
5. Sharma V, Gupta P, Kumar A (2021). Molecular docking studies of bioactive compounds from Murraya koenigii against hypertension-related enzymes. Computational Biology and Chemistry, 91, 107375.
6. Sofowora A. (2006). Medicinal plant and traditional medicine in Africa Spectrum Book Limited Ibadan Nigeria. 289
7. Trease GE, Evans WC. (1989). Pharmacognsy. 11th edn. Brailliar Tiridel Can. Macmillian publishers.
8. Harborne JB. (1998). A Guide to Modern Technique of plant Analysis, 3rd ed. Chapman and Hall, London, 285.
9. Chaves JO, De Souza MC, Da Silva LC, Lachos-Perez D, Torres-Mayanga PC, Machado APDF, Rostagno MA. Extraction of flavonoids from natural sources using modern techniques. Frontiers in Chemistry. 2020; 8:507887. DOI: 10.3389/fchem.2020.507887
10. Pulido KDP, Dulcey AJC, Martínez JHI. New caffeic acid derivative from Tithonia diversifolia (Hemsl.) A. Gray butanolic extract and its antioxidant activity. Food and Chemical Toxicology. 2017; 109:1079-1085
11. Wang H, Zhang H, Li J, Wei J, Zhai R, Peng B, Qian X. A new calibration curve calculation method for absolute quantification of drug metabolizing enzymes in human liver microsomes by stable isotope dilution mass spectrometry. Analytical Methods 2015; 7(14): 5934-5941.
12. Salaudeen IO, Olajuwon MO, Ajala AB, Abdulkareem TO, Adeniyi SA, Jisu SA, Adeyemo GA. Green Synthesis, Characterization and In-vitro Antioxidant Property of Silver Nanoparticles Using the Aqueous Leaf Extract of Justicia carnea. Asian Journal of Biochemistry, Genetics and Molecular Biology. 2021; 7(3): 20-30.
13. Abosede OO, Obiyenwa GK. Green synthesis of copper oxide nanoparticles using Justicia Carnea. Int. J. Chem. Stud. 2022; 10(2): 21-25.
14. Bandeira M, Giovanela M, Roesch-Ely, M., Devine, D. M., & da Silva Crespo, J. (2020). Green synthesis of zinc oxide nanoparticles: A review of the synthesis methodology and mechanism of formation. Sustainable Chemistry and Pharmacy, 15, 10022
15. Mourdikoudis S, Pallares RM, Thanh NT. Characterization techniques for nanoparticles: comparison and complementarity upon studying nanoparticle properties. Nanoscale, 2018; 10(27): 12871-12934.
16. Gumgumjee NM, Hajar AS. Antimicrobial efficacy of Casuarina equisetifolia extracts against some pathogenic microorganisms. Journal of Medicinal Plants Research. 2012; 6(47):5819-25. DOI: 10.5897/JMPR12.741
17. Mohan R, Patel H, Singh J. (2023). Molecular docking and clustering analysis of enzyme inhibitors: A case study on potential anticancer drugs. Computational Biology and Chemistry, 98, 107-115.
18. Morris GM, Huey R, Lindstrom W, Sanner MF, Belew RK, Goodsell DS, Olson AJ. (2009). Auto Dock4 and Auto Dock Tools4: Automated docking with selective receptor flexibility. Journal of Computational Chemistry, 30(16), 2785–2791.
19. Bell LN. Moisture effects on food's chemical stability. Water activity in foods: fundamentals and applications. 2020; 227-253. doi: 10.1002/9780470376454.ch7.
20. Alamgir ANM. Therapeutic use of medicinal plants and their extracts: volume 1. Springer International Publishing AG. 2017; 1(2):30-39.
21. He Y, Wang B, Wen L, Wang F, Yu H, Chen D, Zhang C. Effects of dietary fiber on human health. Food Science and Human Wellness. 2022; 11(1):1-10. https://doi.org/10.1016/j.fshw.2021.07.001.
22. Adelakun SA, Ukwenya VO, Peter AB, Siyanbade AJ, Akinwumiju CO. Therapeutic effects of aqueous extract of bioactive active component of Ageratum conyzoides on the ovarian-uterine and hypophysis-gonadal axis in rat with polycystic ovary syndrome: Histo morphometric evaluation and biochemical assessment. Metabolism Open. 2022; 15:100201. https://doi.org/10.1016/j.metop.2022.100201.
23. Adamski Z, Blythe LL, Milella L, Bufo SA. Biological activities of alkaloids: from toxicology to pharmacology. Toxins. 2020; 12(4):210. https://doi.org/10.3390/toxins12040210.
24. Wang Y, Anesi J, Maier MC, Myers MA, Oqueli E, Sobey CG, Drummond GR, Denton KM. Sympathetic nervous system and atherosclerosis. International Journal of Molecular Sciences. 2023; 24(17):13132.
25. Kumar A, Prasad MNV. Plant-lead interactions: transport, toxicity, tolerance, and detoxification mechanisms. Ecotoxicology and Environmental Safety. 2018; 166:401-418.
26. Joseph DA, Oseni MO, Oseni OA. Biochemical investigations and green synthesis characterization using aqueous extract of Ageratum conyzoides L. leaf. Journal of Biochemicals and Phytomedicine. 2024; 3(2): 9-19. doi: 10.34172/jbp.2024.15.
27. Oseni OA, Seyinde D, Abe TO, Igbe F. Chemical and Some Biochemical Assessments of Various Parts of Passion Fruit (Passiflora edulis Sims) Plant. Asian J. Biol. Sci. 2024; 17(1): 32-40. https://doi.org/10.3923/ajbs.2024.32.40
28. Kaji N, Horiguchi K, Iino S, Nakayama S, Ohwada T, Otani Y, Hori M. Nitric oxide-induced oxidative stress impairs pacemaker function of murine interstitial cells of Cajal during inflammation. Pharmacological Research. 2016; 111:838-848.
29. Zhang Q, Liu Y, Chen Z. Phytochemical profiling of Citrus indicum and its potential therapeutic effects. Phytochemistry Reviews, 2022; 21(4), 1231-1245.
30. Zhang X, Perez-Sanchez H, Lightstone FC. A comprehensive docking and MM/GBSA rescoring study of ligand recognition upon binding antithrombin. Current Topics in Medicinal Chemistry, 2017; 17, 1631-1639.
31. Zhao H, Zhang M, Liu J. Role of oxidative stress in hypertension and its modulation by antioxidants. Oxidative Medicine and Cellular Longevity, 2023; Article ID 7245627.